The layoffs "are not new or unannounced," Universal said.
The 2,050-room Dockside Inn & Suites was supposed to open in March.
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Reopening Orlando and Japan parks with limited capacity has not turned a profit for Universal's theme park division.
Universal didn't specify the number of workers losing their jobs, but did say the cuts did not involve hourly employees.
The park will close on Nov. 2 and reopen on or before March 1.
The ride will include two launches, the fastest hitting 70 mph in 2.4 seconds.
The coaster has been under construction in full view of guests for more than a year.
The two houses will again be open Sept. 26-27 and then from Oct. 3 through Nov. 1.