The layoffs "are not new or unannounced," Universal said.
The layoffs include both union and non-union workers across nearly every department of the Walt Disney World Resort.
720 of the 780 Disney World employees represented by the Actors’ Equity Association have been laid off.
Universal didn't specify the number of workers losing their jobs, but did say the cuts did not involve hourly employees.
In Disney World, the latest layoffs will affect only part-time employees after union negotiations.
Theme park news: New Disneyland store / Disneyland layoff update / Camp Snoopy at Michigan’s Adventure
Downtown Disney is temporarily expanding to a space inside a Disney theme park to sell seasonal merchandise.
Disney has so far disclosed that 2,765 non-union workers in California will be laid off.
The layoffs will affect both Walt Disney World and the Disneyland Resort, with 67 percent of the affected workers being part-timers.
Universal says it now expects the economic impact of the COVID-19 pandemic to stretch into 2021.