The 1973 film was last featured in both Orlando and Hollywood at HHN events in 2016.
It's the first HHN house based around "Texas Chainsaw Massacre" since 2016.
Mazes based on the series will be featured at HHN in both Orlando and Hollywood.
Universal Orlando said the HHN icon's "ominous and unrelenting presence will infiltrate every aspect of the event."
The two houses will again be open Sept. 26-27 and then from Oct. 3 through Nov. 1.
Called Halloween Marathon of Mayhem, the show will utilize infrastructure that Universal debuted last year for its regular nighttime lagoon show, Cinematic Celebration.
It's the first time the 2003 film has been featured at Orlando's Halloween Horror Nights, but Universal Studios Hollywood has based three houses around it at past events.
Universal revealed the scare zones for the event, confirmed the return of the Academy of Villains, and mentioned another “brand-new show that will debut for the first time ever at Halloween Horror Nights.”
Just one day after revealing Jordan Peele’s “Us” for the event, Universal announced another original house named Graveyard Games.