Counties with major parks have seen dramatic increases in COVID-19 hospitalizations since early November.
Mayors of Los Angeles and San Diego were among those supporting a Tier 3 reopening for California's theme parks.
Theme park presidents used words like "arbitrary" and "unachievable" to describe California's guidelines that would allow parks to reopen amid the COVID-19 pandemic.
The release comes after a month of verbal sparring between Disney, the theme park industry and Gov. Gavin Newsom.
The deal comes after Disney agreed to provide free COVID-19 testing for employees, which had been a sticking point for some Disneyland unions.
A team from Gov. Gavin Newsom's administration recently visited Disney World to analyze its parks' COVID-19 safety measures.
In Disney World, the latest layoffs will affect only part-time employees after union negotiations.