At full build-out, the $1 billion resort will offer 10,000 bedrooms across a variety of properties.
Travel agents said guests will learn to live without Magical Express when the service ends in 2022.
The unannounced visits determine whether theme parks are complying with Orange County, Florida's rules on face masks and physical distancing.
Theme park news: Busch Gardens Williamsburg open year-round / Legoland Florida canopy / Renie Bardeau
Busch Gardens Williamsburg will continue hosting limited-capacity weekend events in January and February.
Select rides will be open 6 p.m. to 8 p.m. on Jan. 8 and 9 exclusively for pass members and Fun Card holders.
2021's new attraction lineup ranges from record-setting roller coasters to an immersive hotel experience.
While Central Florida COVID-19 hospitalizations have dropped since their mid-July peak, health experts are still encouraging physical distancing and the use of face masks.